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验证数据展示

  • WB analysis of K-562 using 21687-1-AP

    WB analysis of K-562 using 21687-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21687-1-AP (FBXW12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 21687-1-AP

    IHC staining of human breast cancer using 21687-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 21687-1-AP

    IHC staining of human breast cancer using 21687-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXW12 Polyclonal antibody

FBXW12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21687-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

F box only protein 35, Fbw12, FBXO12, FBXO35, FBXW12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21687-1-AP targets FBXW12 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FBXW12 fusion protein Ag16266 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and WD repeat domain containing 12
别名 F box only protein 35, Fbw12, FBXO12, FBXO35, FBXW12
计算分子量 464 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC101304
基因名称 FBXW12
Gene ID (NCBI) 285231
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6X9E4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.