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FBXO6 Monoclonal antibody

FBXO6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B7F9

Cat No : 67697-1-Ig

Print datasheet

Synonyms

F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6

验证数据展示

  • WB analysis using 67697-1-Ig

    WB analysis using 67697-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67697-1-Ig (FBXO6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67697-1-Ig

    WB analysis of Jurkat using 67697-1-Ig

    WB result of FBXO6 antibody (67697-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FBXO6 transfected Jurkat cells.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, MOLT-4 cells, K-562 cells, U-937 cells, RAW 264.7 cells, HSC-T6 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67697-1-Ig targets FBXO6 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen FBXO6 fusion protein Ag30512 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name F-box protein 6
Synonyms F box only protein 6, F box protein 6, F box/G domain protein 2, FBG2, FBS2, FBX6, Fbx6b, FBXO6
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC020880
Gene Symbol FBXO6
Gene ID (NCBI) 26270
RRIDAB_2882889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO6(F-box only protein 6 ) is also named as FBG2, FBS2, FBX6 and belongs to the F-box protein family. FBXO6 is the substrate recognition component of a Skp1-Cullin1-F-box protein (SCF) ubiquitin E3 ligase complex, recognizing the chitobiose in unfolded N-glycoprotein to target glycoproteins for polyubiquitination and degradation. It can regulate Chk1 ubiquitination and degradation in both normally cycling cells and during replication stress. This protein is mainly detected in the cytoplasm in both cultured cells and in tumor tissues. Its expression is a predictive biomarker of tumor responsiveness to the important anticancer drugs. Positive Fbxo6 expression correlated with early TNM stage and favorable prognosis of NSCLC patients. (PMID:19716789, PMID: 27855403, PMID: 31140586).

实验方案

Product Specific Protocols
WB protocol for FBXO6 antibody 67697-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer

Authors - Pengyu Fan
View Article