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验证数据展示

  • WB analysis using 30162-1-AP

    WB analysis using 30162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30162-1-AP (FBXO44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 30162-1-AP

    IHC staining of mouse brain using 30162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30162-1-AP (FBXO44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30162-1-AP

    IHC staining of mouse brain using 30162-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30162-1-AP (FBXO44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXO44 Polyclonal antibody

FBXO44 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30162-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

DKFZp781J0852, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30162-1-AP targets FBXO44 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag32432

Product name: Recombinant human FBXO44 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 82-122 aa of BC007832

Sequence: LHNPCAEEGFEFWSLDVNGGDEWKVEDLSRDQRKEFPNDQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box protein 44
别名 DKFZp781J0852, F box only protein 44, F box protein 44, F box protein FBX30, F box/G domain protein 3, FBG3, FBX30, Fbx44, FBX6A, FBXO44, Fbxo6a
计算分子量 26 kDa
观测分子量35 kDa
GenBank蛋白编号BC007832
基因名称 FBXO44
Gene ID (NCBI) 93611
RRIDAB_3086253
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4M3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

实验方案

Product Specific Protocols
IHC protocol for FBXO44 antibody 30162-1-APDownload protocol
WB protocol for FBXO44 antibody 30162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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