验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Jurkat cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17806-1-AP targets FBXO42 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | FBXO42 fusion protein Ag12084 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box protein 42 |
| 别名 | F box only protein 42, F box protein 42, F-box only protein 42, FBX42, JFK |
| 计算分子量 | 7171 aa, 78 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC063864 |
| 基因名称 | FBXO42 |
| Gene ID (NCBI) | 54455 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P3S6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO42 is an F-box protein that serves as a substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. It plays a critical role in regulating protein degradation, cell cycle progression, and cellular processes such as chromosome alignment and spindle assembly during mitosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO42 antibody 17806-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |