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FBXO41 Polyclonal antibody

FBXO41 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24519-1-AP

Print datasheet

Synonyms

F box only protein 41, F box protein 41, Fbx41, FBXO41, KIAA1940

验证数据展示

  • WB analysis using 24519-1-AP

    WB analysis using 24519-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24519-1-AP (FBXO41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 24519-1-AP

    IHC staining of mouse brain using 24519-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24519-1-AP (FBXO41 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 24519-1-AP

    IP experiment of mouse brain using 24519-1-AP

    IP result of anti-FBXO41 (IP:24519-1-AP, 4ug; Detection:24519-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 24519-1-AP is FBXO41 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen FBXO41 fusion protein Ag21595 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F-box protein 41
Synonyms F box only protein 41, F box protein 41, Fbx41, FBXO41, KIAA1940
Calculated Molecular Weight 875 aa, 94 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC112202
Gene Symbol FBXO41
Gene ID (NCBI) 150726
RRIDAB_2879586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TF61
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO41 also named as F box only protein 41 is a 875 amino-acid protein, which directly interact with SKP1 and CUL1. FBXO41 is identified as a Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, which as a regulator mediates some protein ubiquitination degradation. FBXO41 consists of F-box domain and has zinc ion binding activity. FBXO41 may have an association with Parkinson's disease.

实验方案

Product Specific Protocols
WB protocol for FBXO41 antibody 24519-1-APDownload protocol
IHC protocol for FBXO41 antibody 24519-1-APDownload protocol
IP protocol for FBXO41 antibody 24519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article
ratWB

iScience

Neuronal F-Box protein FBXO41 regulates synaptic transmission and hippocampal network maturation.

Authors - Ana R A A Quadros
View Article