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验证数据展示

  • WB analysis using 23381-1-AP

    WB analysis using 23381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23381-1-AP (FBXO33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 23381-1-AP

    IF Staining of MCF-7 using 23381-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FBXO33 antibody (23381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

FBXO33 Polyclonal antibody

FBXO33 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23381-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

FBX33, F-box only protein 33, c14_5247, BMND12

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, U2OS cells, mouse lung tissue, rat lung tissue
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23381-1-AP targets FBXO33 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20055

Product name: Recombinant human FBXO33 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 210-555 aa of BC068566

Sequence: DISVLQQQGSLSNTYLSKVDPDGKKIKQIQQLFEEILSNSRQLKWLSCGFMLEIVTPTSLSSLSNAVANTMEHLSLLDNNIPGNSTLITAVELERFVNLHSLALDFCDFTAEMARVLTDSNHVPLQRLSLLVHNVSVMHKSLDNMPNDEHWKALSRKSTSFRVYIMAFDIKSEDMLKILKPSIPLERIHFDSYITCVSGAIVDLISRQYDKFLTHFILMNDVIDTSGFPDLSDNRNEDPLVLLAWRCTKLSLLAIHGYTVWAHNLIAIARLRGSDLKVLEVTEESIDFDQGELADQDVDPVHNLIEQVSLGLGQPWHAVMDIESLSVFTEPNRHFYREMQSFSEDI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box protein 33
别名 FBX33, F-box only protein 33, c14_5247, BMND12
计算分子量 555 aa, 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC068566
基因名称 FBXO33
Gene ID (NCBI) 254170
RRIDAB_3669413
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z6M2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

F-box Only Protein 33 (FBXO33) is a typical member of the F-box protein family with E3 ligase function. FBXO33 can regulate the ubiquitination and solubility of polyglutamine disease proteins, potentially serving as a target for treating type 3 spinocerebellar ataxia. FBXO33 ligase interferes with YB-1-mediated functions by binding and ubiquitinating the multifunctional regulator Y-box-binding protein 1 (YB-1)/dbpB/p50, leading to proteasomal degradation (PMID: 39206900). The calculated molecular weight of FBXO33 is 63 kDa.

实验方案

Product Specific Protocols
IF protocol for FBXO33 antibody 23381-1-APDownload protocol
WB protocol for FBXO33 antibody 23381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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