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验证数据展示

  • IP experiment of PANC-1 using 14570-1-AP

    IP experiment of PANC-1 using 14570-1-AP

    IP result of anti-FBXO27 (IP:14570-1-AP, 4ug; Detection:14570-1-AP 1:600) with PANC-1 cells lysate 1330 ug.

  • WB analysis of Panc-1 cells using 14570-1-AP

    WB analysis of Panc-1 cells using 14570-1-AP

    FBXO27 expression in cells. Each whole cell lysate containing 15 ug protein was analyzed by immunoblotting using FBXO27 (14570-1-AP: 1:2000). Knock-down experiment confirmed that the pointed band showed FBXO27. (Data from Dr. Yukiko Yoshida, Ubiquitin Project, Tokyo Metropolitan Institute of Medical Science.).

  • IHC staining of mouse kidney using 14570-1-AP

    IHC staining of mouse kidney using 14570-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14570-1-AP (FBXO27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14570-1-AP

    IHC staining of mouse kidney using 14570-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14570-1-AP (FBXO27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

FBXO27 Polyclonal antibody

FBXO27 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14570-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA and More (1)

别名

FBG5, F-box only protein 27, F-box/G-domain protein 5, FBX27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPANC-1 cells
Positive IP detected inPANC-1 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14570-1-AP targets FBXO27 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag6157

Product name: Recombinant human FBXO27 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-283 aa of BC030060

Sequence: MGASVSRGRAARVPAPEPEPEEALDLSQLPPELLLVVLSHVPPRTLLGRCRQVCRGWRALVDGQALWLLILARDHGATGRALLHLARSCQSPARNARPCPLGRFCARRPIGRNLIRNPCGQEGLRKWMVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGRIEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLHVTHVFSNIKMGVRFVSFEHRGQDTQFWAGHYGARVTNSSVIVRVRLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box protein 27
别名 FBG5, F-box only protein 27, F-box/G-domain protein 5, FBX27
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC030060
基因名称 FBXO27
Gene ID (NCBI) 126433
RRIDAB_2104422
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NI29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO27 is a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and SCFFBXO27 ubiquitinates glycoproteins in damaged lysosomes will regulate autophagic machinery recruitment (PMID: 28743755).Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes.

实验方案

Product Specific Protocols
IHC protocol for FBXO27 antibody 14570-1-APDownload protocol
IP protocol for FBXO27 antibody 14570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy.

Authors - Yukiko Yoshida
  • KO Validated
View Article
humanWB,IF

Autophagy

TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ

Authors - Chang Woo Chae
View Article