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验证数据展示

  • WB analysis of Jurkat using 68650-1-Ig

    WB analysis of Jurkat using 68650-1-Ig

    WB result of FBXO22 antibody (68650-1-Ig; 1:4000; room temperature for 1.5 hours) with wild-type and FBXO22 knockout Jurkat cells.

  • WB analysis using 68650-1-Ig

    WB analysis using 68650-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68650-1-Ig (FBXO22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

FBXO22 Monoclonal antibody

FBXO22 Monoclonal Antibody for WB, ELISA
Cat No. 68650-1-Ig

产品说明书

CloneNo. 4B8A12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

FBX22, F-box protein FBX22p44, F-box only protein 22, F box protein 22, F box only protein 22

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HEK-293 cells, HepG2 cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68650-1-Ig targets FBXO22 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FBXO22 fusion protein Ag5294 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 F-box protein 22
别名 FBX22, F-box protein FBX22p44, F-box only protein 22, F box protein 22, F box only protein 22
计算分子量 403 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC039024
基因名称 FBXO22
Gene ID (NCBI) 26263
RRIDAB_3670392
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8NEZ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

实验方案

Product Specific Protocols
WB protocol for FBXO22 antibody 68650-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols