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验证数据展示

  • WB analysis using 85936-1-RR

    WB analysis using 85936-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85936-1-RR (FBXO17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 85936-1-RR

    IHC staining of human heart using 85936-1-RR

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 85936-1-RR (FBXO17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXO17 Recombinant monoclonal antibody

FBXO17 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85936-1-RR

产品说明书

CloneNo. 250179E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FBG4, F-box only protein 17, F-box only protein 26, FBX17, FBX26

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, PANC-1 cells, U-251 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85936-1-RR targets FBXO17 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3867

Product name: Recombinant human FBXO17 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-278 aa of BC012385

Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F-box protein 17
别名 FBG4, F-box only protein 17, F-box only protein 26, FBX17, FBX26
计算分子量 278 aa, 31 kDa
观测分子量29 kDa
GenBank蛋白编号BC012385
基因名称 FBXO17
Gene ID (NCBI) 115290
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96EF6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

实验方案

Product Specific Protocols
IHC protocol for FBXO17 antibody 85936-1-RRDownload protocol
WB protocol for FBXO17 antibody 85936-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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