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验证数据展示

  • WB analysis of NCI-H1299 using 68246-1-Ig

    WB analysis of NCI-H1299 using 68246-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68246-1-Ig

    IF Staining of HepG2 using 68246-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (68246-1-Ig, Clone: 1F3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

FBXO17 Monoclonal antibody

FBXO17 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68246-1-Ig

产品说明书

CloneNo. 1F3D3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inNCI-H1299 cells, U2OS cells, A549 cells, SH-SY5Y cells, U-87 MG cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68246-1-Ig targets FBXO17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag3867

Product name: Recombinant human FBXO17 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-278 aa of BC012385

Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 F-box protein 17
别名 F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300
计算分子量 278 aa, 31 kDa
观测分子量29-31 kDa
GenBank蛋白编号BC012385
基因名称 FBXO17
Gene ID (NCBI) 115290
RRIDAB_2935332
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96EF6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

实验方案

Product Specific Protocols
IF protocol for FBXO17 antibody 68246-1-IgDownload protocol
WB protocol for FBXO17 antibody 68246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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