验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, mouse heart tissue, HepG2 cells, mouse lung tissue |
| Positive IHC detected in | human heart tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 3 publications below |
产品信息
12844-1-AP targets FBXO17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag3867 Product name: Recombinant human FBXO17 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-278 aa of BC012385 Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box protein 17 |
| 别名 | FBG4, F-box only protein 17, F-box only protein 26, FBX17, FBX26 |
| 计算分子量 | 278 aa, 31 kDa |
| 观测分子量 | 29-31 kDa |
| GenBank蛋白编号 | BC012385 |
| 基因名称 | FBXO17 |
| Gene ID (NCBI) | 115290 |
| RRID | AB_2877889 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96EF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXO17 antibody 12844-1-AP | Download protocol |
| IHC protocol for FBXO17 antibody 12844-1-AP | Download protocol |
| IF protocol for FBXO17 antibody 12844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients. | ||
J Biol Chem SCFFBXO17 E3 ligase modulates inflammation by regulating proteasomal degradation of glycogen synthase kinase-3β in lung epithelia. | ||
Carcinogenesis Systematically characterize the clinical and biological significances of 1p19q genes in 1p/19q non-codeletion glioma. | ||
J Gene Med Circ_0008717 promotes renal cell carcinoma progression by upregulating FBXO17 via targeting miR-217. | ||
Biosci Biotechnol Biochem F-box protein 17 promotes glioma progression by regulating glycolysis pathway.
| ||
Int J Med Sci FBXO17 Inhibits the Wnt/β-Catenin Pathway and Proliferation of Ishikawa Cells |
