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验证数据展示

  • WB analysis using 32063-1-AP

    WB analysis using 32063-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32063-1-AP (FBXO16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of T-47D using 32063-1-AP

    IP experiment of T-47D using 32063-1-AP

    IP result of anti-FBXO16 (IP:32063-1-AP, 4ug; Detection:32063-1-AP 1:1000) with T-47D cells lysate 1120 ug.

FBXO16 Polyclonal antibody

FBXO16 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32063-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

F box only protein 16, F box protein 16, FBX16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IP detected inT-47D cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32063-1-AP targets FBXO16 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 FBXO16 fusion protein Ag36230 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box protein 16
别名 F box only protein 16, F box protein 16, FBX16
计算分子量 292 aa, 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC074986
基因名称 FBXO16
Gene ID (NCBI) 157574
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8IX29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO16 is a member of the F-box protein family, which functions as a substrate recognition subunit of the SCF (Skp1-Cullin-F-box) E3 ubiquitin ligase complex. High expression levels of FBXO16 are associated with better prognosis in cancer patients. For example, in ovarian cancer, patients with higher FBXO16 expression tend to have a more favorable outcome (PMID: 34333526). This suggests that FBXO16 could serve as a potential biomarker for cancer prognosis and therapeutic targeting.

实验方案

Product Specific Protocols
WB protocol for FBXO16 antibody 32063-1-APDownload protocol
IP protocol for FBXO16 antibody 32063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols