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验证数据展示

  • IHC staining of human breast cancer using 20895-1-AP

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FBXL15 antibody included
  • IHC staining of human breast cancer using 20895-1-AP

    IHC staining of human breast cancer using 20895-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20895-1-AP (FBXL15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FBXL15 antibody included
  • WB analysis of MDA-MB-453s using 20895-1-AP

    WB analysis of MDA-MB-453s using 20895-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20895-1-AP (FBXL15 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

FBXL15 Polyclonal antibody

FBXL15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20895-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® FBXL15 Ready-To-Use IHC Kit. FBXL15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20895-1-AP targets FBXL15 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14949

Product name: Recombinant human FBXL15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC002912

Sequence: MEPPMEPSGGEQEPGAVRFLDLPWEDVLLPHVLNRVPLRQLLRLQRVSRAFRSLVQLHLAGLRRFDAAQVGPQIPRAALARLLRDAEGLQELALAPCHEWLSDEDLVPVLARNPQLRSVALGGCGQLSRRALGALAEGCPRLQRLSLAHCDWVDGLALRGLADRCPALEELDLTACRQLKDEAIVYLAQRRGAGLRSLSLAVNANVGDAAVQELARNCPELHHLDLTGCLRVGSDGVRTLAEYCPVLRSLRVRHCHHVAESSLSRLRKRGVDIDVEPPLHQALVLLQDMAGFAPFVNLQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and leucine-rich repeat protein 15
别名 F box only protein 37, F box/LRR repeat protein 15, Fbl15, FBXL15, FBXO37, JET, PSD
计算分子量 300 aa, 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC002912
基因名称 FBXL15
Gene ID (NCBI) 79176
RRIDAB_2878758
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H469
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXL15, also known as FBXO37, belongs to the FBXL15 family. FBXL15 plays a role in the G2/M transition of the cell cycle, as well as in cellular protein metabolic processes and the positive regulation of the bone morphogenetic proteins signaling pathway (PMID: 38711244). The calculated molecular weight of FBXL15 is 33 kDa.

实验方案

Product Specific Protocols
IHC protocol for FBXL15 antibody 20895-1-APDownload protocol
WB protocol for FBXL15 antibody 20895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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