验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
13934-1-AP targets FBXL14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4975 Product name: Recombinant human FBXL14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-418 aa of BC028132 Sequence: METHISCLFPELLAMIFGYLDVRDKGRAAQVCTAWRDAAYHKSVWRGVEAKLHLRRANPSLFPSLQARGIRRVQILSLRRSLSYVIQGMANIESLNLSGCYNLTDNGLGHAFVQEIGSLRALNLSLCKQITDSSLGRIAQYLKGLEVLELGGCSNITNTGLLLIAWGLQRLKSLNLRSCRHLSDVGIGHLAGMTRSAAEGCLGLEQLTLQDCQKLTDLSLKHISRGLTGLRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFCDKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLELIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMTDSEKEARGDFSPLFTVRTRGSSRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box and leucine-rich repeat protein 14 |
| 别名 | F-box/LRR-repeat protein 14, F-box and leucine-rich repeat protein 14, FBL14 |
| 计算分子量 | 418 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC028132 |
| 基因名称 | FBXL14 |
| Gene ID (NCBI) | 144699 |
| RRID | AB_10547838 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N1E6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXL14(F-box/LRR-repeat protein 14) is also named as FBL14 and belongs to the the F-box protein family,which are characterized by an approximately 40-amino acid F-box motif. It is part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. SNAIL1 is an unstable transcription factor that is phosphorylated by GSK3-beta in the nucleus, which triggers its nuclear export and subsequent ubiquitination and proteasomal degradation. FBXL14 is directed ubiquitination and degradation of SNAIL1 in human and mouse cell lines(PMID:19955572).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FBXL14 antibody 13934-1-AP | Download protocol |
| IHC protocol for FBXL14 antibody 13934-1-AP | Download protocol |
| WB protocol for FBXL14 antibody 13934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7. | ||
Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells |
