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验证数据展示

  • WB analysis using 20251-1-AP

    WB analysis using 20251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20251-1-AP (Asprosin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 20251-1-AP

    IHC staining of human ovary using 20251-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 20251-1-AP

    IHC staining of human ovary using 20251-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 20251-1-AP (FBN1-Specific Antibody) at dilution of 1:50 (under 40x lens).

Asprosin Polyclonal antibody

Asprosin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20251-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

FBN1-Specific, FBN1, Fibrillin 1 , FBN, Fibrillin-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA172 cells
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20251-1-AP targets Asprosin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibrillin 1
别名 FBN1-Specific, FBN1, Fibrillin 1 , FBN, Fibrillin-1
计算分子量 30 kDa
观测分子量32 kDa
GenBank蛋白编号NM_000138
基因名称 Fibrillin 1
Gene ID (NCBI) 2200
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asprosin is a hormone derived from fibrillin-1 that plays a role in regulating glucose metabolism and appetite. It's released by adipose tissue during fasting and stimulates the liver to release glucose into the bloodstream, ensuring energy availability. Asprosin also influences appetite and body weight by acting on neurons in the hypothalamus. It has been linked to various metabolic diseases, including obesity, type 2 diabetes, and polycystic ovary syndrome (PCOS). The detected molecular size is consistent with that in the article PMID: 35079041.

实验方案

Product Specific Protocols
WB protocol for Asprosin antibody 20251-1-APDownload protocol
IHC protocol for Asprosin antibody 20251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols