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验证数据展示

  • WB analysis using 33468-1-AP

    WB analysis using 33468-1-AP

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 33468-1-AP (FAT4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 33468-1-AP

    IHC staining of human colon cancer using 33468-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 33468-1-AP (FAT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 33468-1-AP

    IHC staining of human colon cancer using 33468-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 33468-1-AP (FAT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAT4 Polyclonal antibody

FAT4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33468-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Cadherin family member 14, CDHF14, FAT tumor suppressor homolog 4, FATJ, Fat-like cadherin protein FAT-J

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHGC-27 cells, HUVEC cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33468-1-AP targets FAT4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37862

Product name: Recombinant human FAT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 4200-4504 aa of NM_001291285

Sequence: SVTPDTALSLEGKGRLDYHMSQNEKREYLLRQSLRGAMLEPFGVNSLEVKFRTRSENGVLIHIQESSNYTTVKIKNGKVYFTSDAGIAGKVERNIPEVYVADGHWHTFLIGKNGTATVLSVDRIYNRDIIHPTQDFGGLDVLTISLGGIPPNQAHRDAQTAGFDGCIASMWYGGESLPFSGKHSLASISKTDPSVKIGCRGPNICASNPCWGDLLCINQWYAYRCVPPGDCASHPCQNGGSCEPGLHSGFTCSCPDSHTGRTCEMVVACLGVLCPQGKVCKAGSPAGHVCVLSQGPEEISLPLWA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FAT tumor suppressor homolog 4 (Drosophila)
别名 Cadherin family member 14, CDHF14, FAT tumor suppressor homolog 4, FATJ, Fat-like cadherin protein FAT-J
计算分子量4981aa, 543 kDa
观测分子量 540 kDa
GenBank蛋白编号NM_001291285
基因名称 FAT4
Gene ID (NCBI) 79633
RRIDAB_3742983
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6V0I7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAT4 is a large transmembrane protein with a mass of approximately 530 kDa. It belongs to the cadherin superfamily and contains multiple cadherin repeats EGF-like domains and one laminin G-like domain. FAT4 is expressed widely across tissues with higher expression observed in the kidney liver and cardiovascular system indicating its involvement in diverse physiological processes. This protein plays a role in maintaining cell adhesion and communication.

实验方案

Product Specific Protocols
IHC protocol for FAT4 antibody 33468-1-APDownload protocol
WB protocol for FAT4 antibody 33468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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