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FASTKD2 Polyclonal antibody

FASTKD2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17464-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IF/ICC, IP, ELISA and More (3)

FASTKD 2, FAST kinase domain-containing protein 2, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inCOS7 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17464-1-AP targets FASTKD2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, zebrafish
免疫原 FASTKD2 fusion protein Ag11314 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FAST kinase domains 2
别名 FASTKD 2, FAST kinase domain-containing protein 2, mitochondrial
计算分子量 710 aa, 81 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC001544
基因名称 FASTKD2
Gene ID (NCBI) 22868
RRIDAB_2101119
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FASTKD2 encodes a FAS-activated serine-threonine (FAST) kinase domain 2 which locates in the mitochondrial inner compartment. FASTKD2 is a member of the human FASTK protein family, formed by six architecturally related proteins named FASTK and FASTKD1-5 that localize to mitochondria. The predicted MW of FASTKD2 is 81 kDa, yet the western blot of FASTKD2 revealed a band of ~60 kDa. (PMID: 26370583, PMID: 26990993, PMID: 25683715)

实验方案

Product Specific Protocols
WB protocol for FASTKD2 antibody 17464-1-APDownload protocol
IF protocol for FASTKD2 antibody 17464-1-APDownload protocol
IP protocol for FASTKD2 antibody 17464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,WB

Nat Cell Biol

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Authors - Timo Rey
humanWB

Cell Metab

A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Authors - Jason D Arroyo
humanIF

Proc Natl Acad Sci U S A

Cytosolic N6AMT1-dependent translation supports mitochondrial RNA processing

Authors - Mads M Foged
humanIF

Nucleic Acids Res

FASTKD1 and FASTKD4 have opposite effects on expression of specific mitochondrial RNAs, depending upon their endonuclease-like RAP domain.

Authors - Erik Boehm
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
humanWB, IF, RIP

Cell Rep

Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis.

Authors - Hana Antonicka
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