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FAS/CD95 Monoclonal antibody
FAS/CD95 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1G7F7
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, K-562 cells, U-937 cells |
| Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60196-1-Ig targets FAS/CD95 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, Zebrafish |
| Immunogen | FAS/CD95 fusion protein Ag3718 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Fas (TNF receptor superfamily, member 6) |
| Synonyms | ALPS1A, APO 1, Apo 1 antigen, APT1, CD95, FAS, FAS/CD95, FAS1, FASLG receptor, FASTM, TNFRSF6 |
| Calculated Molecular Weight | 35-38 kDa |
| Observed Molecular Weight | 38-45 kDa |
| GenBank Accession Number | BC012479 |
| Gene Symbol | FAS |
| Gene ID (NCBI) | 355 |
| RRID | AB_10892669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25445 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAS/CD95 antibody 60196-1-Ig | Download protocol |
| IHC protocol for FAS/CD95 antibody 60196-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesis.
| ||
Biochim Biophys Acta Down-regulation of islet amyloid polypeptide expression induces death of human annulus fibrosus cells via mitochondrial and death receptor pathways. | ||
J Physiol Biochem miR-128-3p regulates 3T3-L1 adipogenesis and lipolysis by targeting Pparg and Sertad2. | ||
iScience Loss of Adipose Growth Hormone Receptor in Mice Enhances Local Fatty Acid Trapping and Impairs Brown Adipose Tissue Thermogenesis. | ||
Chemosphere Possible involvement of Fas/FasL-dependent apoptotic pathway in α-bisabolol induced cardiotoxicity in zebrafish embryos. | ||
Food Funct Hawk tea prevents high-fat diet-induced obesity in mice by activating the AMPK/ACC/SREBP1c signaling pathways and regulating the gut microbiota. |
