验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HL-60 cells, K-562 cells, human liver tissue, mouse brain tissue, PC-3 cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, mouse cerebellum tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy FARSB Ready-To-Use IHC Kit. FARSB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16341-1-AP targets FARSB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9522 Product name: Recombinant human FARSB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-589 aa of BC017783 Sequence: DHSRITVNTRNIFIECTGTDFTKAKIVLDIIVTMFSEYCENQFTVEAAEVVFPNGKSHTFPELAYRKEMVRADLINKKVGIRETPENLAKLLTRMYLKSEVIGDGNQIEIEIPPTRADIIHACDIVEDAAIAYGYNNIQMTLPKTYTIANQFPLNKLTELLRHDMAAAGFTEALTFALCSQEDIADKLGVDISATKAVHISNPKTAEFQVARTTLLPGLLKTIAANRKMPLPLKLFEISDIVIKDSNTDVGAKNYRHLCAVYYNKNPGFEIIHGLLDRIMQLLDVPPGEDKGGYVIKASEGPAFFPGRCAEIFARGQSVGKLGVLHPDVITKFELTMPCSSLEINIGPFL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phenylalanyl-tRNA synthetase, beta subunit |
| 别名 | Phenylalanine--tRNA ligase beta subunit, PheHB, HSPC173, FRSB, FARSLB |
| 计算分子量 | 589 aa, 66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC017783 |
| 基因名称 | FARSB |
| Gene ID (NCBI) | 10056 |
| RRID | AB_2102515 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSD9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FARSB antibody 16341-1-AP | Download protocol |
| IHC protocol for FARSB antibody 16341-1-AP | Download protocol |
| IP protocol for FARSB antibody 16341-1-AP | Download protocol |
| WB protocol for FARSB antibody 16341-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Chem Soc Oxidation-Controlled, Strain-Promoted Tellurophene-Alkyne Cycloaddition (OSTAC): A Bioorthogonal Tellurophene-Dependent Conjugation Reaction | ||
Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. | ||
Neural Regen Res A novel therapeutic target for peripheral nerve injury-related diseases: aminoacyl-tRNA synthetases. | ||
Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA | ||
Int J Mol Sci FARSB Facilitates Hepatocellular Carcinoma Progression by Activating the mTORC1 Signaling Pathway |
