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- KD/KO Validated
FARSA Polyclonal antibody
FARSA Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells, Hela cells |
Planning an IHC experiment? We recommend our IHCeasy FARSA Ready-To-Use IHC Kit. FARSA primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:4000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18121-1-AP targets FARSA in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, RIP, ELISA |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | FARSA fusion protein Ag12647 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phenylalanyl-tRNA synthetase, alpha subunit |
| Synonyms | CML33, FARS, FARSA, FARSL, FARSLA, FRSA, PheHA, PheRS |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC006495 |
| Gene Symbol | FARSA |
| Gene ID (NCBI) | 2193 |
| RRID | AB_2102509 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y285 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FARSA antibody 18121-1-AP | Download protocol |
| IHC protocol for FARSA antibody 18121-1-AP | Download protocol |
| IF protocol for FARSA antibody 18121-1-AP | Download protocol |
| IP protocol for FARSA antibody 18121-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. | ||
Clin Genet FARS1-related disorders caused by bi-allelic mutations in cytosolic phenylalanyl-tRNA synthetase genes: Look beyond the lungs! | ||
Nat Commun Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.
| ||
Autoimmun Rev Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase | ||
Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA |
