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验证数据展示

  • WB analysis using 85638-4-RR

    WB analysis using 85638-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85638-4-RR

    Affinity and Kinetic Characterization of 85638-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85638-4-RR against Human FARP1 were performed. The affinity constant is 1.18 nM.

FARP1 Recombinant monoclonal antibody

FARP1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85638-4-RR

产品说明书

CloneNo. 243132G6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CDEP, Chondrocyte-derived ezrin-like protein, FERM, ARHGEF and pleckstrin domain-containing protein 1, FERM, RhoGEF and pleckstrin domain-containing protein 1, PH domain-containing family C member 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, A375 cells
Planning an IHC experiment? We recommend our IHCeasy® FARP1 Ready-To-Use IHC Kit. FARP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85638-4-RR targets FARP1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21225

Product name: Recombinant human FARP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 347-539 aa of BC071592

Sequence: VLDYVKEGGHKKVQFERKHSKIHSIRSLASQPTELNSEVLEQSQQSTSLTFGEGAESPGGQSCRRGKEPKVSAGEPGSHPSPAPRRSPAGNKQADGAASAPTEEEEEVVKDRTQQSKPQPPQPSTGSLTGSPHLSELSVNSQGGVAPANVTLSPNLSPDTKQASPLISPLLNDQACPRTDDEDEGRRKRFPTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
别名 CDEP, Chondrocyte-derived ezrin-like protein, FERM, ARHGEF and pleckstrin domain-containing protein 1, FERM, RhoGEF and pleckstrin domain-containing protein 1, PH domain-containing family C member 2
计算分子量 1076 aa, 122 kDa
观测分子量122-130 kDa
GenBank蛋白编号BC071592
基因名称 FARP1
Gene ID (NCBI) 10160
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4F1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.

实验方案

Product Specific Protocols
WB protocol for FARP1 antibody 85638-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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