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验证数据展示

  • WB analysis using 17167-1-AP

    WB analysis using 17167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17167-1-AP (FANK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

FANK1 Polyclonal antibody

FANK1 Polyclonal Antibody for WB, ELISA
Cat No. 17167-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

UNQ6504/PRO21382, HSD13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17167-1-AP targets FANK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FANK1 fusion protein Ag10639 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibronectin type III and ankyrin repeat domains 1
别名 UNQ6504/PRO21382, HSD13
计算分子量 345 aa, 38 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC024189
基因名称 FANK1
Gene ID (NCBI) 92565
RRIDAB_3669264
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC84
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FANK1 (Fibronectin type 3 and ankyrin repeat domains protein 1), also known as HSD13. It is expected to be located in the nucleus. And mostly restricted to testis (PMID: 17604233). Fibronectin type III and ankyrin repeat domains 1(Fank1) is a nuclear protein expressed during the transition from the meiotic phase to the haploid phase of spermatogenesis. While little is known about the function of Fank1 during spermatogenesis, gene ontology analysis suggests that it may be a transcription factor. H. Wang explored the role of Fank1 using the yeast two-hybrid system to screen for cellular proteins that may interact with Fank1. In this screen, they identified the Jun activation domain-binding protein 1 (Jab1) as a potential Fank1-interacting protein (PMID: 20978819).

实验方案

Product Specific Protocols
WB protocol for FANK1 antibody 17167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols