验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
10215-1-AP targets FANCG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FANCG fusion protein Ag0249 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Fanconi anemia, complementation group G |
| 别名 | XRCC9, Protein FACG, Fanconi anemia group G protein, FAG, DNA repair protein XRCC9 |
| 计算分子量 | 69 kDa |
| 观测分子量 | 65-68 kDa |
| GenBank蛋白编号 | BC000032 |
| 基因名称 | FANCG |
| Gene ID (NCBI) | 2189 |
| RRID | AB_2231541 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15287 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FANCG antibody 10215-1-AP | Download protocol |
| IF protocol for FANCG antibody 10215-1-AP | Download protocol |
| IP protocol for FANCG antibody 10215-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
J Cancer Significant value of XRCC2 and XRCC9 expression in the prognosis of human ovarian carcinoma. | ||
Clin Transl Oncol Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study. |
