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验证数据展示

  • WB analysis using 83946-4-RR

    WB analysis using 83946-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83946-4-RR (FANCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 83946-4-RR

    WB analysis of HeLa using 83946-4-RR

    WB result of FANCD2 antibody (83946-4-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCD2 transfected HeLa cells.

  • IF Staining of HeLa using 83946-4-RR

    IF Staining of HeLa using 83946-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FANCD2 antibody (83946-4-RR, Clone: 241048G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of HeLa using 83946-4-RR

    FC experiment of HeLa using 83946-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug FANCD2 Recombinant antibody (83946-4-RR, Clone:241048G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83946-4-RR

    Affinity and Kinetic Characterization of 83946-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83946-4-RR against Human FANCD2 were performed. The affinity constant is below 1 pM.

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FANCD2 Recombinant monoclonal antibody

FANCD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83946-4-RR

产品说明书

CloneNo. 241048G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

241048G8, FA4, FACD, FAD, FAD2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, Jurkat cells, U2OS cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83946-4-RR targets FANCD2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29460

Product name: Recombinant human FANCD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-127 aa of NM_001018115

Sequence: MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fanconi anemia, complementation group D2
别名 241048G8, FA4, FACD, FAD, FAD2
计算分子量 164 kDa
观测分子量150-160 kDa
GenBank蛋白编号NM_001018115
基因名称 FANCD2
Gene ID (NCBI) 2177
RRIDAB_3671526
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BXW9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Amongst the FA genes, Fanconi Anemia Group D2 Protein (FANCD2) is the most evolutionarily conserved gene, demonstrating a high sequence similarity from lower eukaryotes to humans. Upon activation, Fanconi anemia group D2 protein (FANCD2) is monoubiquitinated at K561 and works in cooperation with other FA and non-FA proteins to repair DNA damage. In regards to the FA pathway, monoubiquitinated or activated FANCD2 is often portrayed as the functional representative for the functions of activated FA signaling. (PMID: 28825622)

实验方案

Product Specific Protocols
IF protocol for FANCD2 antibody 83946-4-RRDownload protocol
WB protocol for FANCD2 antibody 83946-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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