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验证数据展示

  • WB analysis of HL-60 using 19543-1-AP

    WB analysis of HL-60 using 19543-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19543-1-AP

    IHC staining of human testis using 19543-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 19543-1-AP

    IHC staining of human testis using 19543-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19543-1-AP (FANCD2-phospho(S330) antibody) at dilution of 1:50 (under 10x lens).

Phospho-FANCD2 (Ser330) Polyclonal antibody

Phospho-FANCD2 (Ser330) Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19543-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Phospho-FANCD2 (Ser330), Protein FACD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19543-1-AP targets Phospho-FANCD2 (Ser330) in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fanconi anemia, complementation group D2
别名 FA D2, FA4, FACD, FAD, FAD2, FANCD, FANCD2, Phospho-FANCD2 (Ser330), Protein FACD2
计算分子量 166 kDa
观测分子量 150-155 kDa
GenBank蛋白编号NM_033084
基因名称 FANCD2
Gene ID (NCBI) 2177
RRIDAB_10640576
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXW9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FANCD2, also named as FACD and FACD2, is required for maintenance of chromosomal stability. FANCD2 promotes accurate and efficient pairing of homologs during meiosis. FANCD2 is involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. It may participate in S phase and G2 phase checkpoint activation upon DNA damage. It promotes BRCA2/FANCD1 loading onto damaged chromatin. FANCD2 may also be involved in B-cell immunoglobulin isotype switching. Defects in FANCD2 are a cause of Fanconi anemia (FA) which is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. The antibody is to phospho-Ser(330) of FANCD2.

实验方案

Product Specific Protocols
WB protocol for Phospho-FANCD2 (Ser330) antibody 19543-1-APDownload protocol
IHC protocol for Phospho-FANCD2 (Ser330) antibody 19543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols