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FANCC Polyclonal antibody

FANCC Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16973-1-AP

Print datasheet

Synonyms

FA3, FAC, FACC, FANCC, Fanconi anemia group C protein, Protein FACC

验证数据展示

  • WB analysis of HepG2 using 16973-1-AP

    WB analysis of HepG2 using 16973-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16973-1-AP (FANCC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 16973-1-AP

    IHC staining of human brain using 16973-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16973-1-AP (FANCC antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 16973-1-AP

    IHC staining of human brain using 16973-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16973-1-AP (FANCC antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, K-562 cells, Jurkat cells, NIH/3T3 tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16973-1-AP targets FANCC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen FANCC fusion protein Ag9423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fanconi anemia, complementation group C
Synonyms FA3, FAC, FACC, FANCC, Fanconi anemia group C protein, Protein FACC
Calculated Molecular Weight 558 aa, 63 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC015748
Gene Symbol FANCC
Gene ID (NCBI) 2176
RRIDAB_2231524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FANCC antibody 16973-1-APDownload protocol
IHC protocol for FANCC antibody 16973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols