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  • Featured Product
  • KD/KO Validated

FANCA Polyclonal antibody

FANCA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11975-1-AP

Print datasheet

Synonyms

FA, FA H, FA1, FAA, FACA, FAH, FANCA, FANCH, Fanconi anemia group A protein, Protein FACA

验证数据展示

  • WB analysis of HeLa cells using 11975-1-AP

    WB analysis of HeLa cells using 11975-1-AP

    WB results of FANCA (11975-1-AP, 1:500) with si-control and si-FANCA transfected HeLa cells.

  • IHC staining of human colon cancer using 11975-1-AP

    IHC staining of human colon cancer using 11975-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon cancer using 11975-1-AP

    IHC staining of human colon cancer using 11975-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11975-1-AP (FANCA Antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis using 11975-1-AP

    WB analysis using 11975-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11975-1-AP (FANCA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11975-1-AP targets FANCA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen FANCA fusion protein Ag2598 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fanconi anemia, complementation group A
Synonyms FA, FA H, FA1, FAA, FACA, FAH, FANCA, FANCH, Fanconi anemia group A protein, Protein FACA
Calculated Molecular Weight 163 kDa
Observed Molecular Weight 163 kDa
GenBank Accession NumberBC008979
Gene Symbol FANCA
Gene ID (NCBI) 2175
RRIDAB_2101786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15360
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FANCA, also named as FAA, FACA and FANCH, is a DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

实验方案

Product Specific Protocols
WB protocol for FANCA antibody 11975-1-APDownload protocol
IHC protocol for FANCA antibody 11975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Struct Mol Biol

Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.

Authors - Xinlin Xu
  • KO Validated
View Article
mouseWB

EMBO Mol Med

WNT inhibition creates a BRCA-like state in Wnt-addicted cancer.

Authors - Amanpreet Kaur
View Article
humanWB

Cell Rep

C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair

Authors - Zenan Zhou
View Article
humanIHC,IF,IP

Gene

Identification of rare heterozygous missense mutations in FANCA in esophageal atresia patients using next-generation sequencing.

Authors - Yu Feng
View Article