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FAM98B Polyclonal antibody

FAM98B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22251-1-AP

Print datasheet

Synonyms

FAM98B, Protein FAM98B

验证数据展示

  • WB analysis using 22251-1-AP

    WB analysis using 22251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22251-1-AP

    IHC staining of human breast cancer using 22251-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.

  • IHC staining of human breast cancer using 22251-1-AP

    IHC staining of human breast cancer using 22251-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 22251-1-AP

    IF Staining of HepG2 using 22251-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 22251-1-AP (FAM98B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 22251-1-AP

    WB analysis using 22251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, Raji cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22251-1-AP is FAM98B Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen FAM98B fusion protein Ag17747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 98, member B
Synonyms FAM98B, Protein FAM98B
Calculated Molecular Weight 330 aa, 37 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC093898
Gene Symbol FAM98B
Gene ID (NCBI) 283742
RRIDAB_2879049
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ52LJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM98B antibody 22251-1-APDownload protocol
IHC protocol for FAM98B antibody 22251-1-APDownload protocol
IF protocol for FAM98B antibody 22251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols