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验证数据展示

  • WB analysis using 22251-1-AP

    WB analysis using 22251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22251-1-AP

    IHC staining of human breast cancer using 22251-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.

  • IHC staining of human breast cancer using 22251-1-AP

    IHC staining of human breast cancer using 22251-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22251-1-AP (FAM98B Antibody) at dilution of 1:50.

  • WB analysis using 22251-1-AP

    WB analysis using 22251-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22251-1-AP (FAM98B antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 22251-1-AP

    IF Staining of HepG2 using 22251-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FAM98B antibody (22251-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

FAM98B Polyclonal antibody

FAM98B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22251-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Protein FAM98B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, Raji cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22251-1-AP targets FAM98B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 FAM98B fusion protein Ag17747 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 98, member B
别名 Protein FAM98B
计算分子量 330 aa, 37 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC093898
基因名称 FAM98B
Gene ID (NCBI) 283742
RRIDAB_2879049
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ52LJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM98B antibody 22251-1-APDownload protocol
IHC protocol for FAM98B antibody 22251-1-APDownload protocol
IF protocol for FAM98B antibody 22251-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Commun Biol

DDX1 is required for non-spliceosomal splicing of tRNAs but not of XBP1 mRNA

Authors - Teruhiko Suzuki
View Article