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验证数据展示

  • IHC staining of mouse lung using 26413-1-AP

    IHC staining of mouse lung using 26413-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26413-1-AP ( FAM92B/CIBAR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse lung using 26413-1-AP

    IF Staining of mouse lung using 26413-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse lung using 26413-1-AP

    IF Staining of mouse lung using 26413-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using FAM92B antibody (26413-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of HeLa using 26413-1-AP

    WB analysis of HeLa using 26413-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26413-1-AP (FAM92B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FAM92B/CIBAR2 Polyclonal antibody

FAM92B/CIBAR2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 26413-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA and More (1)

别名

FAM92B, Protein FAM92B, CIBAR2, CBY1-interacting BAR domain-containing protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, mouse small intestine tissue
Positive IF-P detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

26413-1-AP targets FAM92B/CIBAR2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 FAM92B/CIBAR2 fusion protein Ag24802 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 92, member B
别名 FAM92B, Protein FAM92B, CIBAR2, CBY1-interacting BAR domain-containing protein 2
计算分子量 304 aa, 35 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC111944
基因名称 FAM92B
Gene ID (NCBI) 339145
RRIDAB_2880506
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZTR7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM92B (family with sequence similarity 92, member B, also named CIBAR2) is a transmembrane protein that plays a crucial role in various cellular processes, including membrane trafficking and cell adhesion. The protein is predicted to be intracellular and is involved in ciliogenesis, likely through the recruitment and fusion of endosomal vesicles at distal appendages during early stages of ciliogenesis.

实验方案

Product Specific Protocols
WB protocol for FAM92B/CIBAR2 antibody 26413-1-APDownload protocol
IF protocol for FAM92B/CIBAR2 antibody 26413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Life Sci Alliance

ciBAR1 loss in mice causes laterality defects, pancreatic degeneration, and altered glucose tolerance

Authors - Eunice N Kim
View Article