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验证数据展示

  • WB analysis using 26032-1-AP

    WB analysis using 26032-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26032-1-AP (FAM83B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brown disease using 26032-1-AP

    IHC staining of human brown disease using 26032-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26032-1-AP (FAM83B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM83B antibody included
  • IHC staining of human brown disease using 26032-1-AP

    IHC staining of human brown disease using 26032-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26032-1-AP (FAM83B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM83B antibody included

FAM83B Polyclonal antibody

FAM83B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26032-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C6orf143

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FAM83B Ready-To-Use IHC Kit. FAM83B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26032-1-AP targets FAM83B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag22951

Product name: Recombinant human FAM83B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 653-1011 aa of BC112275

Sequence: NQQTENLLKRRSFPLFDNSKANLDPGNSKHYVYSTLTRNRVRQPEKPKEDLLKSSKSMHNVTHNLEEDEEEVTKRNSPSGTTTKSVSIAALLDVNKEESNKELASKKEVKGSPSFLKKGSQKLRSLLSLTPDKKENLSKNKAPAFYRLCSSSDTLVSEGEENQKPKKSDTKVDSSPRRKHSSSSNSQGSIHKSKEDVTVSPSQEINAPPDENKRTPSPGPVESKFLERAGDASAPRFNTEQIQYRDSREINAVVNPERRPTSSPRPTSSELLRSHSTDRRVYSRFEPFCKIESSIQPTSNMPNTSINRPEIKSATMGNSYGRSSPLLNYNTGVYRSYQPNENKFRGFMQKFGNFIHKNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 83, member B
别名 C6orf143
计算分子量 1011 aa, 115 kDa
观测分子量115 kDa
GenBank蛋白编号BC112275
基因名称 FAM83B
Gene ID (NCBI) 222584
RRIDAB_3085837
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T0W9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM83B, also known as C6orf143, belongs to the FAM83 family. FAM83B is an oncogene and promotes cell transformation through activating various pathway in different kinds of tumors. FAM83B over expressed in several kinds of malignant tumors, such as breast cancer, cervical cancer, endometrial cancer, pancreatic ductal adenocarcinoma, etc. Overexpression of FAM83B could promote the malignant biological behaviors of these tumors (PMID: 33423038, 36690987). The calculated molecular weight of FAM83B is 115 kDa.

实验方案

Product Specific Protocols
IHC protocol for FAM83B antibody 26032-1-APDownload protocol
WB protocol for FAM83B antibody 26032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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