验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, DU 145 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20618-1-AP targets FAM83A in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14680 Product name: Recombinant human FAM83A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-294 aa of BC007828 Sequence: MSRSRHLGKIRKRLEDVKSQWVRPARADFSDNESARLATDALLDGGSEAYWRVLSQEGEVDFLSSVEAQYIQAQAREPPCPPDTLGGAEAGPKGLDSSSLQSGTYFPVASEGSEPALLHSWASAEKPYLKEKSSATVYFQTVKHNNIRDLVRRCITRTSQNISIRSVEGEIYCAKSGRKFTGQIREKFIISDWRFVLSGSYSFTWLCGHVHRNILSKFTGQAVELFDEEFRHLYASSKPVMGLKSPRLVAPVPPGAAPANGRLSSSSGSASDRTSSNPFSGRSAGSHPGTRTDG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 83, member A |
| 别名 | BJ TSA 9, Protein FAM83A, TSGP, Tumor antigen BJ TSA 9, Tumor antigen BJ-TSA-9 |
| 计算分子量 | 434 aa, 47 kDa |
| 观测分子量 | 47-58 kDa |
| GenBank蛋白编号 | BC007828 |
| 基因名称 | FAM83A |
| Gene ID (NCBI) | 84985 |
| RRID | AB_2878708 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86UY5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM83A antibody 20618-1-AP | Download protocol |
| WB protocol for FAM83A antibody 20618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer | ||
Int J Mol Sci Involvement of MicroRNA-1-FAM83A Axis Dysfunction in the Growth and Motility of Lung Cancer Cells.
| ||
Clin Epigenetics Aberrant epigenetic and transcriptional events associated with breast cancer risk. | ||
J Transl Med FAM83A promotes proliferation and metastasis via Wnt/β-catenin signaling in head neck squamous cell carcinoma.
| ||
BMC Cancer Lovastatin inhibits erythroleukemia progression through KLF2-mediated suppression of MAPK/ERK signaling |
