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验证数据展示

  • WB analysis using 33752-1-AP

    WB analysis using 33752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33752-1-AP (FAM82A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 33752-1-AP

    WB analysis using 33752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33752-1-AP (FAM82A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of U2OS using 33752-1-AP

    IP experiment of U2OS using 33752-1-AP

    IP result of anti-FAM82A1 (IP:33752-1-AP, 4ug; Detection:33752-1-AP 1:2000) with U2OS cells lysate 760 ug.

  • IHC staining of human urothelial carcinoma using 33752-1-AP

    IHC staining of human urothelial carcinoma using 33752-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 33752-1-AP (FAM82A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 33752-1-AP

    IHC staining of human urothelial carcinoma using 33752-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 33752-1-AP (FAM82A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 33752-1-AP

    IF Staining of SH-SY5Y using 33752-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using FAM82A1 antibody (33752-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U2OS using 33752-1-AP

    IF Staining of U2OS using 33752-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using FAM82A1 antibody (33752-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM82A1 Polyclonal antibody

FAM82A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 33752-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

BLOCK18, FAM82A, hRMD-2, Protein FAM82A1, Regulator of microtubule dynamics protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, U2OS cells, human testis tissue, rat liver
Positive IP detected inU2OS cells
Positive IHC detected inhuman urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33752-1-AP targets FAM82A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag40522

Product name: Recombinant human FAM82A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-125 aa of BC024243

Sequence: KVRKPGIAMKLPEFLSLGNTFNSITLQDEIHDDQGTTVIFQERQLQILEKLNELLTNMEELKEEIRFLKEAIPKLEEYIQDELGGKITVHKISPQHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 82, member A1
别名 BLOCK18, FAM82A, hRMD-2, Protein FAM82A1, Regulator of microtubule dynamics protein 2
观测分子量45 kDa
GenBank蛋白编号BC024243
基因名称 FAM82A1
Gene ID (NCBI) 151393
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96LZ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM82A1 is a crucial cellular signaling regulatory protein that has recently gained attention for its role in tumorigenesis. Research indicates that it serves as an important regulatory subunit of protein phosphatase 2A (PP2A), participating in the precise regulation of cell cycle progression and apoptosis by influencing key signaling pathways such as JNK/c-Jun. In various cancers (e.g., glioblastoma, lung cancer), FAM82A1 often exhibits abnormally high expression, which is closely associated with accelerated cancer cell proliferation, inhibited apoptosis, and poor patient prognosis. Its cancer-promoting function is primarily achieved by sustaining cancer cell survival, making FAM82A1 not only a potential tumor biomarker but also a promising new target for anti-cancer therapy worthy of further exploration.

实验方案

Product Specific Protocols
IF protocol for FAM82A1 antibody 33752-1-APDownload protocol
IHC protocol for FAM82A1 antibody 33752-1-APDownload protocol
IP protocol for FAM82A1 antibody 33752-1-APDownload protocol
WB protocol for FAM82A1 antibody 33752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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