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验证数据展示

  • WB analysis of mouse liver using 21038-1-AP

    WB analysis of mouse liver using 21038-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21038-1-AP (FAM78A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21038-1-AP

    IHC staining of human tonsillitis using 21038-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

  • IHC staining of human tonsillitis using 21038-1-AP

    IHC staining of human tonsillitis using 21038-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 21038-1-AP (FAM78A Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 21038-1-AP

    IF Staining of HepG2 using 21038-1-AP

    Immunofluorescent analysis of HepG2 cells using 21038-1-AP (FAM78A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FAM78A Polyclonal antibody

FAM78A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21038-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Protein FAM78A, C9orf59

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, human liver tissue, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21038-1-AP targets FAM78A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15366

Product name: Recombinant human FAM78A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 5-57 aa of BC049388

Sequence: QSIGGKARVFREGITVIDVKASIDPVPTSIDESSSVVLRYRTPHFRASAQVVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 78, member A
别名 Protein FAM78A, C9orf59
计算分子量 283 aa, 32 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC049388
基因名称 FAM78A
Gene ID (NCBI) 286336
RRIDAB_10973677
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JUQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM78A antibody 21038-1-APDownload protocol
IHC protocol for FAM78A antibody 21038-1-APDownload protocol
IF protocol for FAM78A antibody 21038-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols