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验证数据展示

  • WB analysis using 20863-1-AP

    WB analysis using 20863-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20863-1-AP (FAM76A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20863-1-AP

    IHC staining of mouse testis using 20863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20863-1-AP (FAM76A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20863-1-AP

    IHC staining of mouse testis using 20863-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20863-1-AP (FAM76A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM76A Polyclonal antibody

FAM76A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20863-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

FAM76A, Protein FAM76A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20863-1-AP targets FAM76A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14904

Product name: Recombinant human FAM76A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-227 aa of BC025768

Sequence: QRKHLSSSSRAGHQEKEQYSRLSGGGHYNSQKTLSTSSIQNEIPKKKSKFESITTNGDSFSPDLALDSPGTDHFVIIAQLKEEVAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 76, member A
别名 FAM76A, Protein FAM76A
计算分子量 307 aa, 35 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC025768
基因名称 FAM76A
Gene ID (NCBI) 199870
RRIDAB_10693681
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM76A is a member of FAM76 family. It is reported that FAM76A may be regulated by WTAP. FAM76A is highly expressed in the WTAP high-expression group and also expressed at low levels in the WTAP low-expression group, which can be confirmed by TCGA data (PMID: 32998774).

实验方案

Product Specific Protocols
IHC protocol for FAM76A antibody 20863-1-APDownload protocol
WB protocol for FAM76A antibody 20863-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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