验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, U2O2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29811-1-AP targets MIGA2/FAM73B in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | MIGA2/FAM73B fusion protein Ag30577 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 73, member B |
| 别名 | MIGA2, FAM73B, PSEC0112, Protein FAM73B, Mitoguardin 2 |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC009114 |
| 基因名称 | MIGA2/FAM73B |
| Gene ID (NCBI) | 84895 |
| RRID | AB_3086170 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L4E1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MIGA2 (mitoguardin-2) also named as FAM73B, is a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), and identified as a lipid transporter. MIGA2 localizes to contact sites between mitochondria and the ER or mitochondria and lipid droplets (LDs) (PMID: 36282247). Based on its presence in many tissues, MIGA2 is likely critical for lipid and energy homeostasis in a wide spectrum of cell types (PMID: 31628041).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIGA2/FAM73B antibody 29811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |