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验证数据展示

  • WB analysis using 29811-1-AP

    WB analysis using 29811-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29811-1-AP (FAM73B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 29811-1-AP

    IHC staining of human placenta using 29811-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29811-1-AP (MIGA2/FAM73B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIGA2/FAM73B antibody included
  • IHC staining of human placenta using 29811-1-AP

    IHC staining of human placenta using 29811-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29811-1-AP (MIGA2/FAM73B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MIGA2/FAM73B antibody included

MIGA2/FAM73B Polyclonal antibody

MIGA2/FAM73B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29811-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FAM73B, MIGA2, C9orf54, Mitoguardin 2, Protein FAM73B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, U2O2 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® MIGA2/FAM73B Ready-To-Use IHC Kit. MIGA2/FAM73B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29811-1-AP targets MIGA2/FAM73B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30577

Product name: Recombinant human FAM73B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 62-180 aa of BC009114

Sequence: AHQLKRRRRRKKQVGPEMGGEQLGTVPLPILLARKVPSVKKGYSSRRVQSPSSKSNDTLSGISSIEPSKHSGSSHSVASMMAVNSSSPTAACSGLWDARGMEESLTTSDGNAESLYMQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 73, member B
别名 FAM73B, MIGA2, C9orf54, Mitoguardin 2, Protein FAM73B
观测分子量65-70 kDa
GenBank蛋白编号BC009114
基因名称 MIGA2/FAM73B
Gene ID (NCBI) 84895
RRIDAB_3086170
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L4E1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MIGA2 (mitoguardin-2) also named as FAM73B, is a mitochondrial protein at contacts with the ER and/or lipid droplets (LDs), and identified as a lipid transporter. MIGA2 localizes to contact sites between mitochondria and the ER or mitochondria and lipid droplets (LDs) (PMID: 36282247). Based on its presence in many tissues, MIGA2 is likely critical for lipid and energy homeostasis in a wide spectrum of cell types (PMID: 31628041).

实验方案

Product Specific Protocols
IHC protocol for MIGA2/FAM73B antibody 29811-1-APDownload protocol
WB protocol for MIGA2/FAM73B antibody 29811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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