验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17015-1-AP targets FAM65B in WB, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | FAM65B fusion protein Ag9991 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 65, member B |
| 别名 | C6orf32, DIFF40, DIFF48, FAM65B, KIAA0386, PL48, Protein FAM65B, RIPOR2 |
| 计算分子量 | 118 kDa |
| 观测分子量 | 115 kDa, 66 kDa |
| GenBank蛋白编号 | BC001232 |
| 基因名称 | FAM65B |
| Gene ID (NCBI) | 9750 |
| RRID | AB_10640810 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y4F9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM65B is also named as C6orf32, DIFF48, KIAA0386, PL48 and belongs to the FAM65 family.It has 2 isoforms produced by alternative splicing.Isoform 2 play a role in promoting myogenic cell differentiation, cytoskeletal rearrangement and filopodia formation(PMID:17150207). FAM65B is expressed in mononuclear cells, not in myotubes, upon induction of differentiation and cellular fusion, and expression is upregulated during human myoblast differentiation at both the mRNA and protein levels(PMID:17150207).This antibody is specific to the isoform of 119 kDa of FAM65B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM65B antibody 17015-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing. | ||
J Cell Sci Front Signal-Dependent Accumulation of RHOA Inhibitor FAM65B at Leading Edges Polarizes Neutrophils.
| ||
FASEB J Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation. |
