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验证数据展示

  • WB analysis using 19849-1-AP

    WB analysis using 19849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19849-1-AP (FAM50A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat colon using 19849-1-AP

    IHC staining of rat colon using 19849-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19849-1-AP (FAM50A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM50A antibody included
  • IHC staining of rat kidney using 19849-1-AP

    IHC staining of rat kidney using 19849-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 19849-1-AP (FAM50A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM50A antibody included

FAM50A Polyclonal antibody

FAM50A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19849-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Protein HXC-26, Protein HXC 26, Protein FAM50A, HXC26, HXC 26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inrat colon tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FAM50A Ready-To-Use IHC Kit. FAM50A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19849-1-AP targets FAM50A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FAM50A fusion protein Ag13907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 50, member A
别名 Protein HXC-26, Protein HXC 26, Protein FAM50A, HXC26, HXC 26
计算分子量 325 aa, 39 kDa
观测分子量40 kDa
GenBank蛋白编号BC000028
基因名称 FAM50A
Gene ID (NCBI) 9130
RRIDAB_10641032
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14320
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM50A belongs to the FAM50 family. It is highly conserved in length and sequence across different species. It is a basic protein containing a nuclear localization signal, and may function as a DNA-binding protein or a transcriptional factor.

实验方案

Product Specific Protocols
WB protocol for FAM50A antibody 19849-1-APDownload protocol
IHC protocol for FAM50A antibody 19849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols