验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse brain tissue, human placenta tissue, human testis tissue, mouse testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
产品信息
20127-1-AP targets FAM49B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | FAM49B fusion protein Ag13939 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 49, member B |
| 别名 | BM 009, FAM49B, L1, Protein FAM49B |
| 计算分子量 | 324 aa, 37 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | BC003599 |
| 基因名称 | FAM49B |
| Gene ID (NCBI) | 51571 |
| RRID | AB_10638431 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NUQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM49B antibody 20127-1-AP | Download protocol |
| IHC protocol for FAM49B antibody 20127-1-AP | Download protocol |
| IF protocol for FAM49B antibody 20127-1-AP | Download protocol |
| IP protocol for FAM49B antibody 20127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.
| ||
Genes (Basel) Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells.
| ||
Mol Immunol FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner.
| ||
Elife CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake |
