- Featured Product
- KD/KO Validated
FAM49B Polyclonal antibody
FAM49B Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse brain tissue, human placenta tissue, human testis tissue, mouse testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
产品信息
The immunogen of 20127-1-AP is FAM49B Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | FAM49B fusion protein Ag13939 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | family with sequence similarity 49, member B |
| Synonyms | BM 009, FAM49B, L1, Protein FAM49B |
| Calculated Molecular Weight | 324 aa, 37 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC003599 |
| Gene Symbol | FAM49B |
| Gene ID (NCBI) | 51571 |
| RRID | AB_10638431 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUQ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM49B antibody 20127-1-AP | Download protocol |
| IHC protocol for FAM49B antibody 20127-1-AP | Download protocol |
| IF protocol for FAM49B antibody 20127-1-AP | Download protocol |
| IP protocol for FAM49B antibody 20127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.
| ||
Genes (Basel) Characterization and Functional Study of FAM49B Reveals Its Effect on Cell Proliferation in HEK293T Cells.
| ||
Mol Immunol FAM49B, restrained by miR-22, relieved hepatic ischemia/reperfusion injury by inhibiting TRAF6/IKK signaling pathway in a Rac1-dependent manner.
|
