验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, Jurkat cells |
| Positive IHC detected in | mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24419-1-AP targets FAM48A in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag19875 Product name: Recombinant human FAM48A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 482-701 aa of BC030686 Sequence: TPQQTSSFLKSPTPPPSSKPSSIPRKSSVDLNQVSMLSPAALSPASSSQRTTATQVMANSAGLNFINVVGSVCGAQALMSGSNPMLGCNTGAITPAGINLSGLLPSGGLLPNALPSAMQAASQAGVPFGLKNTSSLRPLNLLQLPGGSLIFNTLQQQQQQLSQFTPQQPQQPTTCSPQQPGEQGSEQGSTSQEQALSAQQAAVINLTGVGSFMQSQAAVL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 48, member A |
| 别名 | C13, C13orf19, FAM48A, FP757, p38 interacting protein, P38IP, Protein FAM48A |
| 计算分子量 | 779 aa, 86 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | BC030686 |
| 基因名称 | FAM48A |
| Gene ID (NCBI) | 55578 |
| RRID | AB_2879537 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NEM7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM48A also named as C13orf19 or SUPT20H is a 779 amino acid protein, which belongs to the SPT20 family. FAM48A is required for MAP kinase p38 activation during gastrulation and for starvation-induced ATG9A trafficking during autophagy. FAM48A localizes in the nucleus and is highly expressed in testis, moderately in brain and pituitary gland.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM48A antibody 24419-1-AP | Download protocol |
| WB protocol for FAM48A antibody 24419-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |






