验证数据展示
经过测试的应用
| Positive IHC detected in | human prostate cancer tissue, rat spleen tissue, mouse spleen tissue, mouse kidney tissue, human spleen tissue, rat kidney tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy® FAM46C Ready-To-Use IHC Kit. FAM46C primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
25038-1-AP targets FAM46C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag19147 Product name: Recombinant human FAM46C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-67 aa of BC131726 Sequence: MAEESSCTRDCMSFSVLNWDQVSRLHEVLTEVVPIHGRGNFPTLEITLKDIVQTVRSRLEEAGIKVH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 46, member C |
| 别名 | EC:2.7.7.19, Non-canonical poly(A) polymerase FAM46C, TENT5C, Terminal nucleotidyltransferase 5C |
| 计算分子量 | 391 aa, 45 kDa |
| GenBank蛋白编号 | BC131726 |
| 基因名称 | FAM46C |
| Gene ID (NCBI) | 54855 |
| RRID | AB_2879863 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VWP2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM46C antibody 25038-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep The C-terminal region of TENT5 proteins drives ER-associated mRNA polyadenylation via FNDC3 interaction. | ||
Cancer Sci Biallelic loss of FAM46C triggers tumor growth with concomitant activation of Akt signaling in multiple myeloma cells.
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