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验证数据展示

  • WB analysis using 32785-1-AP

    WB analysis using 32785-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32785-1-AP (FAM3D antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 32785-1-AP

    IHC staining of mouse colon using 32785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32785-1-AP (FAM3D antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 32785-1-AP

    IHC staining of mouse colon using 32785-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32785-1-AP (FAM3D antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM3D Polyclonal antibody

FAM3D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32785-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Oit1, Oncoprotein-induced protein 1, Protein EF-7, Protein FAM3D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse colon tissue, rat colon tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32785-1-AP targets FAM3D in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2694

Product name: Recombinant Mouse Oit1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-223 aa of NM_146050.2

Sequence: YTSFSRKTIRLPRWLGITPKDIQTPKSKCGLSKICPNNFFAFKISSGAANVVGPSMCFEDEIIMSPVRNNVGRGLNVALVNGSTGQVMKKDSFDMYSGDPQLLLNFLTEIPDSTLVLVASYDDPGTKMNDKIKTLFSNLGSSYAKQLGFRDSWVFVGAKDLKSKSPYEQFLKNNPETNKYDGWPELLELEGCVPRKVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oncoprotein induced transcript 1
别名 Oit1, Oncoprotein-induced protein 1, Protein EF-7, Protein FAM3D
计算分子量25 kDa
观测分子量25 kDa
GenBank蛋白编号NM_146050.2
基因名称 Oit1
Gene ID (NCBI) 18300
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP97805
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM3D (family with sequence similarity 3, member D) is a protein that belongs to the FAM3 family, which is a novel cytokine-like gene family identified in 2002. This family comprises four members: FAM3A, FAM3B, FAM3C, and FAM3D. FAM3D is a gut-secreted protein that is regulated by nutritional status. It has a predicted secondary structure of a four-helix bundle, which is a common feature in many other cytokines.

实验方案

Product Specific Protocols
IHC protocol for FAM3D antibody 32785-1-APDownload protocol
WB protocol for FAM3D antibody 32785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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