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验证数据展示

  • WB analysis using 19570-1-AP

    WB analysis using 19570-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19570-1-AP (FAM32A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 19570-1-AP

    IF Staining of U2OS using 19570-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FAM32A antibody (19570-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

FAM32A Polyclonal antibody

FAM32A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19570-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CGI-144, OTAG12, OTAG-12, Ovarian tumor-associated gene 12, Protein FAM32A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19570-1-AP targets FAM32A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13761

Product name: Recombinant human FAM32A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-112 aa of BC000639

Sequence: MEAYEQVQKGPLKLKGVAELGVTKRKKKKKDKDKAKLLEAMGTSKKNEEEKRRGLDKRTPAQAAFEKMQEKRQMERILKKASKTHKQRVEDFNRHLDTLTEHYDIPKVSWTK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 32, member A
别名 CGI-144, OTAG12, OTAG-12, Ovarian tumor-associated gene 12, Protein FAM32A
计算分子量 112 aa, 13 kDa
观测分子量13 kDa
GenBank蛋白编号BC000639
基因名称 FAM32A
Gene ID (NCBI) 26017
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y421
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM32A (family with sequence similarity 32 member) is also known as ovarian tumor associated gene-12 (OTAG-12, OTAG12) and its expression is suppressed in ovarian cancer. FAM32A is involved in increased cell proliferation and suppresses apoptosis (PMID: 21339736). FAM32A protein is a 13 kDa protein that consists of 112 amino acids and is predominantly located in the nucleus (PMID: 39730183).

实验方案

Product Specific Protocols
IF protocol for FAM32A antibody 19570-1-APDownload protocol
WB protocol for FAM32A antibody 19570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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