验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19570-1-AP targets FAM32A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag13761 Product name: Recombinant human FAM32A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-112 aa of BC000639 Sequence: MEAYEQVQKGPLKLKGVAELGVTKRKKKKKDKDKAKLLEAMGTSKKNEEEKRRGLDKRTPAQAAFEKMQEKRQMERILKKASKTHKQRVEDFNRHLDTLTEHYDIPKVSWTK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 32, member A |
| 别名 | CGI-144, OTAG12, OTAG-12, Ovarian tumor-associated gene 12, Protein FAM32A |
| 计算分子量 | 112 aa, 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC000639 |
| 基因名称 | FAM32A |
| Gene ID (NCBI) | 26017 |
| RRID | AB_3741962 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y421 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM32A (family with sequence similarity 32 member) is also known as ovarian tumor associated gene-12 (OTAG-12, OTAG12) and its expression is suppressed in ovarian cancer. FAM32A is involved in increased cell proliferation and suppresses apoptosis (PMID: 21339736). FAM32A protein is a 13 kDa protein that consists of 112 amino acids and is predominantly located in the nucleus (PMID: 39730183).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM32A antibody 19570-1-AP | Download protocol |
| WB protocol for FAM32A antibody 19570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
