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FAM29A Polyclonal antibody

FAM29A Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16933-1-AP

Print datasheet

Synonyms

Dgt6, DGT6, HAUS6, KIAA1574, FAM29A, HAUS6, KIAA1574, RP11 296P7.3

验证数据展示

  • WB analysis of Jurkat using 16933-1-AP

    WB analysis of Jurkat using 16933-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16933-1-AP (FAM29A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16933-1-AP

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16933-1-AP targets FAM29A in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen FAM29A fusion protein Ag10421 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 29, member A
Synonyms Dgt6, DGT6, HAUS6, KIAA1574, FAM29A, HAUS6, KIAA1574, RP11 296P7.3
Calculated Molecular Weight 955 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC010632
Gene Symbol FAM29A
Gene ID (NCBI) 54801
RRIDAB_1939544
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM29A antibody 16933-1-APDownload protocol
IF protocol for FAM29A antibody 16933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article