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验证数据展示

  • WB analysis of Jurkat using 16933-1-AP

    WB analysis of Jurkat using 16933-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16933-1-AP (FAM29A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16933-1-AP

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 16933-1-AP

    IF Staining of HeLa using 16933-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM29A antibody (16933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM29A Polyclonal antibody

FAM29A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16933-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA1574, HAUS6, HAUS augmin-like complex subunit 6, DGT6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16933-1-AP targets FAM29A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag10421

Product name: Recombinant human FAM29A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-354 aa of BC010632

Sequence: MEENRTKEPIQMDVEHREVLPESLPVLHNQREFSMADFLLETTVSDFGQSHLTEEKVISDCECVPQKHVLTSHIDEPPTQNQSDLLNKKVICKQDLECLAFTKLSETSRMETFSPAVGNRIDVMGGSEEEFMKILDHLEVSCNKPSTNKTMLWNSFQISIGISSKSFKDNDFGILHETLPEEVGHLSFNSSSSSEANFKLEPNSPMHGGTLLEDVVGGRQTTPESDFNLQALRSRYEALKKSLSKKREESYLSNSQTPERHKPELSPTPQNVQTDDTLNFLDTCDLHTEHIKPSLRTSIGERKRSLSPLIKFSPVEQRLRTTIACSLGELPNLKEEDILNKSLDAKEPPSDLTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 29, member A
别名 KIAA1574, HAUS6, HAUS augmin-like complex subunit 6, DGT6
计算分子量 955 aa, 109 kDa
观测分子量 109 kDa
GenBank蛋白编号BC010632
基因名称 FAM29A
Gene ID (NCBI) 54801
RRIDAB_1939544
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4H7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for FAM29A antibody 16933-1-APDownload protocol
WB protocol for FAM29A antibody 16933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
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