验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HepG2 cells, HuH-7 cells, MDA-MB-453 cells, SH-SY5Y cells, TT cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31914-1-AP targets FAM222A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36045 Product name: Recombinant human C12orf34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 191-325 aa of BC037221 Sequence: LPPSNLPSIHSLLYQLNQQCQAPGAAPPACQGMAIPHPSPAKHGPVPSFPSMAYSAAAGLPDCRKGTELGQGATQALTLAGAAKPAGYADSGLDYLLWPQKPPPPPPQPLRAYSGSTVASKSPEACGGRAYERAS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 12 open reading frame 34 |
| 别名 | Protein FAM222A, FLJ14721, Family With Sequence Similarity 222 Member A, Chromosome 12 Open Reading Frame 34, C12orf34 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC037221 |
| 基因名称 | C12orf34 |
| Gene ID (NCBI) | 84915 |
| RRID | AB_3670141 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5U5X8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Family with sequence similarity 222 member A (FAM222A, also known as C12orf34) is associated with the progress of Alzheimer's disease (PMID: 31964863; 37092222). It is also a novel key determinant of endothelial biology and angiogenesis (PMID: 37942611).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM222A antibody 31914-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
