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FAM20A Polyclonal antibody

FAM20A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25258-1-AP

Print datasheet

Synonyms

FAM20A, FP2747, Protein FAM20A

验证数据展示

  • IHC staining of human colon cancer using 25258-1-AP

    IHC staining of human colon cancer using 25258-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25258-1-AP (FAM20A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse lung using 25258-1-AP

    WB analysis of mouse lung using 25258-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25258-1-AP (FAM20A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25258-1-AP is FAM20A Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen FAM20A fusion protein Ag19494 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 20, member A
Synonyms FAM20A, FP2747, Protein FAM20A
Calculated Molecular Weight 541 aa, 61 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC136686
Gene Symbol FAM20A
Gene ID (NCBI) 54757
RRIDAB_2879991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MK3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM20A antibody 25258-1-APDownload protocol
IHC protocol for FAM20A antibody 25258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols