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验证数据展示

  • WB analysis using 21078-1-AP

    WB analysis using 21078-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21078-1-AP (FAM190B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 21078-1-AP

    IHC staining of mouse kidney using 21078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21078-1-AP (FAM190B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 21078-1-AP

    IHC staining of mouse kidney using 21078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21078-1-AP (FAM190B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM190B Polyclonal antibody

FAM190B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21078-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

KIAA1128, Gcap14, Protein FAM190B, Protein GCAP14 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, U2OS cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21078-1-AP targets FAM190B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FAM190B fusion protein Ag15281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1128
别名 KIAA1128, Gcap14, Protein FAM190B, Protein GCAP14 homolog
计算分子量 834 aa, 94 kDa
观测分子量 63 kDa
GenBank蛋白编号BC030528
基因名称 KIAA1128
Gene ID (NCBI) 54462
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7U1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM190B (Family with sequence similarity 190, member B), also known as Gcap14, CCSER2, KIAA1128, NPD012, is a CC domain-containing protein with multiple isoforms generated by alternative splicing that functions as an MAP. FAM190B is a microtubule plus-end-tracking protein and as a regulator of microtubule dynamics during neurodevelopment (PMID: 36795749).

实验方案

Product Specific Protocols
WB protocol for FAM190B antibody 21078-1-APDownload protocol
IHC protocol for FAM190B antibody 21078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols