验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells |
Positive IHC detected in | human hepatocirrhosis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20911-1-AP targets FAM185A in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | FAM185A fusion protein Ag15041 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | family with sequence similarity 185, member A |
别名 | FAM185A, Protein FAM185A |
计算分子量 | 393 aa, 42 kDa |
观测分子量 | 30 kDa, 42 kDa |
GenBank蛋白编号 | BC029175 |
基因名称 | FAM185A |
Gene ID (NCBI) | 222234 |
RRID | AB_10732807 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8N0U4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for FAM185A antibody 20911-1-AP | Download protocol |
IHC protocol for FAM185A antibody 20911-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |