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验证数据展示

  • IHC staining of human heart using 26868-1-AP

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM175B antibody included
  • IHC staining of human heart using 26868-1-AP

    IHC staining of human heart using 26868-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26868-1-AP (FAM175B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FAM175B antibody included
  • WB analysis of HL-60 using 26868-1-AP

    WB analysis of HL-60 using 26868-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26868-1-AP (FAM175B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FAM175B Polyclonal antibody

FAM175B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26868-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Abraxas brother protein 1, ABRO1, BRISC complex subunit Abro1, FAM175B, KIAA0157, Protein FAM175B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HEK-293 cells, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FAM175B Ready-To-Use IHC Kit. FAM175B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26868-1-AP targets FAM175B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 FAM175B fusion protein Ag22121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 175, member B
别名 Abraxas brother protein 1, ABRO1, BRISC complex subunit Abro1, FAM175B, KIAA0157, Protein FAM175B
计算分子量 415 aa, 47 kDa
观测分子量 50 kDa
GenBank蛋白编号BC008999
基因名称 FAM175B
Gene ID (NCBI) 23172
RRIDAB_2880663
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15018
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM175B antibody 26868-1-APDownload protocol
IHC protocol for FAM175B antibody 26868-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

A bacterial RING ubiquitin ligase triggering stepwise degradation of BRISC via TOLLIP-mediated selective autophagy manipulates host inflammatory response

Authors - Xinming Pan
View Article