验证数据展示
经过测试的应用
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32901-1-AP targets FAM173A in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag37690 Product name: Recombinant human FAM173A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 43-235 aa of BC001181 Sequence: QPGFRRVPLRLQVPYVGASARQVEHVLSLLRGRPGKTVDLGSGDGRIVLAAHRCGLRPAVGYELNPWLVALARLHAWRAGCAGSVCYRRKDLWKVSLRDCRNVSVFLAPSVLPLLEDKLRTELPAGARVVSGRFPLPTWQPVTAVGEGLDRVWAYDVPEGGQAGEAASSRIPIQAAPGPSSAPIPGGLISQAS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 173, member A |
| 别名 | Adenine nucleotide translocase lysine N-methyltransferase, ANTKMT , ANT-KMT, C16orf24, EC:2.1.1.- |
| 计算分子量 | 235 aa, 25 kDa |
| GenBank蛋白编号 | BC001181 |
| 基因名称 | FAM173A |
| Gene ID (NCBI) | 65990 |
| RRID | AB_3742781 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9BQD7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANTKMT (FAM173A) encodes adenine nucleotide translocase lysine methyltransferase, a protein-lysine methyltransferase that carries out peptidyl-lysine trimethylation. It is a member of the 7BS protein lysine methyltransferase and ANT/ATPSC lysine N-methyltransferase families. The protein localizes to the mitochondrion, mitochondrial membrane, cytosol, and membrane, placing it in compartments relevant to mitochondrial energy metabolism.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAM173A antibody 32901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
