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  • Featured Product
  • KD/KO Validated

FAM171A2 Polyclonal antibody

FAM171A2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20836-1-AP

Print datasheet

Synonyms

FAM171A2, Protein FAM171A2

验证数据展示

  • WB analysis of mouse liver using 20836-1-AP

    WB analysis of mouse liver using 20836-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20836-1-AP (FAM171A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20836-1-AP

    IHC staining of mouse brain using 20836-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20836-1-AP (FAM171A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20836-1-AP is FAM171A2 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen FAM171A2 fusion protein Ag14909 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 171, member A2
Synonyms FAM171A2, Protein FAM171A2
Calculated Molecular Weight 826 aa, 87 kDa
Observed Molecular Weight 65-75 kDa
GenBank Accession NumberBC030200
Gene Symbol FAM171A2
Gene ID (NCBI) 284069
RRIDAB_2878748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA8MVW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM171A2 antibody 20836-1-APDownload protocol
IHC protocol for FAM171A2 antibody 20836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC,IF

Sci Adv

The FAM171A2 gene is a key regulator of progranulin expression and modifies the risk of multiple neurodegenerative diseases.

Authors - Wei Xu
  • KD Validated
View Article